0033. Hypothermia protects brain mitochondrial function from hypoxia in sepsis

Methods Mice (C57/bl6) were injected intraperitoneally with lipopolysaccharide (LPS) (5 mg/kg; n = 15) or saline (0.01 ml/g; n = 6). Six hours later, the cerebral cortex was exposed and mitochondrial function assessed using the inherent fluorescence of flavin adenine dinucleotide (FAD). The mice spontaneously breathed a sequence of 21% oxygen (room air), 100%, 21%, 15%, 21% and 10% for 5 minutes each. Seven of the endotoxic mice were maintained at their original body temperature post-LPS injection, while eight were maintained at 37°C using a homeothermic heating mat. Systemic oxygenation was assessed using pulse oximetry. Statistical analysis was performed by selecting areas around the veins and arteries in order to ratio the fluorescence intensity of arteries over veins. The difference in the ratio between groups was compared using a one way ANOVA followed by independent sample t-tests on IBM SPSS 22.